Ence ExperimentsTo verify the binding between ARL11 and partner proteins, HEK-293T cells growing on glass cover slips were transiently cotransfected with plasmids MedChemExpress AZP-531 containing YFP1-CRABP2, YFP1-In-Frame cDNA Libraryplasmids encoding HA-ARL11 served as control. Immunoprecipitation was performed using the anti-HA antibody agarose beads as described above, and the AZP-531 price membranes were probed with antiCRABP2 (Sigma-Aldrich, C6873:1:2,000), anti-PGAM1 (SigmaAldrich, Sab1100295; 1:2,500), or anti-GFP N-terminal (SigmaAldrich, G1544; 1:1,000) antibody.Table S3 DNA sequences of clones identified as putativeARL11 binders. (DOC)Table S4 Clones identified as putative ARL11 binders.(DOC)Supporting InformationKozak sequences from position 17 to 32 plus first 3 codons of 174 random in-frame cDNA library clones. (DOC)Table SAcknowledgmentsWe thank Stephanie Garza and Virginia Hurley for secretarial assistance and Kim-Anh T. Vu for computerized graphic design of the figures.Author ContributionsConceived and designed the experiments: SL BC. Performed the experiments: SL IL. Analyzed the data: SL YJ DM BC. Wrote the paper: BC.Kozak sequence analysis of 174 random inframe cDNA library clones. (DOC)Table S
There is strong evidence that pregnant women and infants are at increased risk of severe illness following infection with influenza virus [1]. Hospitalization for respiratory illness related to seasonal influenza is more frequent in pregnant than in non pregnant women [2,3], and the risk of death in pregnant women increased during influenza pandemics compared to non-pandemic years [4]. The emergence of A/H1N1 influenza infection in Mexico and in Australia in early 2009 raised further awareness and concernworldwide. In June 2009, World Health Organization raised the pandemic alert level to the highest level of 6 [5]. In August 2009, researchers from the Centers for Disease Control and Prevention reported that 6/45 (13 ) patients who died from 2009 A/H1N1 influenza between mid-April and mid-June were pregnant women [6]. The disproportionately increased risk of mortality due to A/ H1N1 2009 influenza infection in pregnant women was confirmed by the Centers for Disease Control and Prevention survey [6]. Pregnant women have been therefore designated as a top priority group to receive the pandemic A/H1N1 2009 influenza vaccinePandemic Influenza 2009 Vaccine and Pregnancy[7?1]. In France, the vaccination campaign was launched in November 2009; a single dose of a non-adjuvanted A/H1N1 2009 influenza vaccine was recommended for all pregnant women after the first trimester [11]. Most of available data are issued from retrospective studies and prospective cohort studies are still lacking to better understand how A/H1N1 2009 influenza pandemic affects pregnant women. Furthermore, whereas some studies have shown safety, immunogenicity and effectiveness of seasonal flu vaccination in pregnant women [4,12,13], additional data are still needed to assess the safety and efficacy of maternal vaccination during pandemic period. In the context of the A/H1N1 2009 influenza pandemic, we planned a prospective study conducted in the general population of pregnant women to assess the incidence, the maternal-fetal impact of 2009 influenza pandemic, and the effectiveness and the safety of maternal vaccination. When it appeared that the pandemic level expected by public health services would be not 12926553 achieved, the objectives of the study were redefined to assess: 1) the incidence of laboratory.Ence ExperimentsTo verify the binding between ARL11 and partner proteins, HEK-293T cells growing on glass cover slips were transiently cotransfected with plasmids containing YFP1-CRABP2, YFP1-In-Frame cDNA Libraryplasmids encoding HA-ARL11 served as control. Immunoprecipitation was performed using the anti-HA antibody agarose beads as described above, and the membranes were probed with antiCRABP2 (Sigma-Aldrich, C6873:1:2,000), anti-PGAM1 (SigmaAldrich, Sab1100295; 1:2,500), or anti-GFP N-terminal (SigmaAldrich, G1544; 1:1,000) antibody.Table S3 DNA sequences of clones identified as putativeARL11 binders. (DOC)Table S4 Clones identified as putative ARL11 binders.(DOC)Supporting InformationKozak sequences from position 17 to 32 plus first 3 codons of 174 random in-frame cDNA library clones. (DOC)Table SAcknowledgmentsWe thank Stephanie Garza and Virginia Hurley for secretarial assistance and Kim-Anh T. Vu for computerized graphic design of the figures.Author ContributionsConceived and designed the experiments: SL BC. Performed the experiments: SL IL. Analyzed the data: SL YJ DM BC. Wrote the paper: BC.Kozak sequence analysis of 174 random inframe cDNA library clones. (DOC)Table S
There is strong evidence that pregnant women and infants are at increased risk of severe illness following infection with influenza virus [1]. Hospitalization for respiratory illness related to seasonal influenza is more frequent in pregnant than in non pregnant women [2,3], and the risk of death in pregnant women increased during influenza pandemics compared to non-pandemic years [4]. The emergence of A/H1N1 influenza infection in Mexico and in Australia in early 2009 raised further awareness and concernworldwide. In June 2009, World Health Organization raised the pandemic alert level to the highest level of 6 [5]. In August 2009, researchers from the Centers for Disease Control and Prevention reported that 6/45 (13 ) patients who died from 2009 A/H1N1 influenza between mid-April and mid-June were pregnant women [6]. The disproportionately increased risk of mortality due to A/ H1N1 2009 influenza infection in pregnant women was confirmed by the Centers for Disease Control and Prevention survey [6]. Pregnant women have been therefore designated as a top priority group to receive the pandemic A/H1N1 2009 influenza vaccinePandemic Influenza 2009 Vaccine and Pregnancy[7?1]. In France, the vaccination campaign was launched in November 2009; a single dose of a non-adjuvanted A/H1N1 2009 influenza vaccine was recommended for all pregnant women after the first trimester [11]. Most of available data are issued from retrospective studies and prospective cohort studies are still lacking to better understand how A/H1N1 2009 influenza pandemic affects pregnant women. Furthermore, whereas some studies have shown safety, immunogenicity and effectiveness of seasonal flu vaccination in pregnant women [4,12,13], additional data are still needed to assess the safety and efficacy of maternal vaccination during pandemic period. In the context of the A/H1N1 2009 influenza pandemic, we planned a prospective study conducted in the general population of pregnant women to assess the incidence, the maternal-fetal impact of 2009 influenza pandemic, and the effectiveness and the safety of maternal vaccination. When it appeared that the pandemic level expected by public health services would be not 12926553 achieved, the objectives of the study were redefined to assess: 1) the incidence of laboratory.

Ence ExperimentsTo verify the binding between ARL11 and partner proteins, HEK-

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