Nt was shown to lower the histopathological changes, for instance hyperplasia of follicular cells and related hypertrophic modifications (Fig. 5A). Also, MOK pharmacopuncture at 0.three and 1.5 mg/kg drastically improved the follicular size (P0.001, respectively) compared with that of the control group (Fig. 5B).HWANG et al: 275-51-4 custom synthesis Effects OF MOK PHARMACOPUNCTURE ON HYPOTHYROIDISMFigure four. Effects of MOK pharmacopuncture around the adjustments of serological parameters in S-Methylglutathione MedChemExpress PTU-induced hyperthyroidism rats. MOK pharmacopuncture was subcutaneously administered when daily for 2 weeks, as well as the levels of (A) glucose, (B) triglyceride, (C) total cholesterol, (D) LDL-cholesterol, (E) AST, and (F) ALT in the sera of rats had been measured by automatic blood biochemical analyzer. Data are presented as imply typical deviation (n=5 per each group). P0.05, P0.01, and P0.001 vs. standard; #P0.05, ##P0.01, and ###P0.001 vs. handle. Typical, normal group; PTU+Vehicle, control group; PTU+Low MOK, MOK 0.3 ml/kg-treated group in control; PTU+High MOK, MOK 1.5 mg/kg-treated group in manage; and PTU+LT4, L-Thyroxine 0.5 mg/kg-treated group as a reference drug.Figure five. Effects of MOK pharmacopuncture around the histopathological alterations of thyroid tissues in PTU-induced hypothyroidism rats. MOK pharmacopuncture was subcutaneously administered as soon as everyday for two weeks, and thyroid glands were isolated in the rats. (A) Thyroid tissues were stained with H E dye. Morphological adjustments were observed by a microscope at x200 in original magnification. Arrow: Follicle membrane, and f: Follicle. (B) The mean of relative follicular sizes to typical group have been measured in PTU-induced hypothyroidism rats. Information are presented as mean standard deviation (n=5 per each group). P0.001 vs. normal; ###P0.001 vs. control. Standard, standard group; PTU+Vehicle, handle group; PTU+Low MOK, MOK 0.three ml/kg-treated group in control; PTU+High MOK, MOK 1.five mg/kg-treated group in control; and PTU+LT4, L-Thyroxine 0.5 mg/kg-treated group as a reference drug.Effect of MOK pharmacopuncture on oxidation in the liver and brain of hypothroidism rats. To investigate the effect of MOK pharmacopuncture on oxidative damage in hypothyroidism, we measured the levels on the antioxidant substance GSH within the liver tissues of hyperthyroidism rats and also the expression of the antioxidant enzymes SOD and CAT in both liver and brain tissues. As shown in Fig. 6A, the level ofGSH was significantly (P0.05) reduced in the liver tissues of PTUinduced hypothyroidism rats and substantially increased in the rats treated with MOK pharmacopuncture at 0.three (P0.01) and 1.five mg/kg (P0.05). Next, the expression of SOD protein was improved in hypothyroidism rats and drastically decreased in both liver (P0.05; Fig. 6B) and brain tissues (P0.01; Fig. 6C) compared with that with the control group afterEXPERIMENTAL AND THERAPEUTIC MEDICINE 16: 310-320,Figure six. Impact of MOK pharmacopuncture on the oxidation in liver and brain tissues of PTU-induced hypothyroidism rats. MOK pharmacopuncture was subcutaneously administered once daily for 2 weeks, and also the levels of (A) GSH from the liver of rats by ELISA have been measured. The expression of CAT and SOD2 in the (B) liver and (C) brain tissues working with western blot. Information are presented as imply typical deviation (n=5 per every single group). P0.05 vs. regular; # P0.05, ##P0.01, and ###P0.001 vs. handle. Typical, standard group; PTU+Vehicle, control group; PTU+Low MOK, MOK 0.three ml/kg-treated group in manage; PTU+High MOK, MOK 1.five.