Mg/kg-treated group in handle; and PTU+LT4, L-Thyroxine 0.five mg/kg-treated group as a reference drug.MOK Pharmacopuncture at 1.5 mg/kg. CAT Chlorazol Black E medchemexpress expression was substantially (P0.05) decreased in liver and brain tissues. The hypothyroidisminduced decrease in CAT was significantly enhanced inside the liver (P0.001) and brain tissues (P0.05) by MOK pharmacopuncture at 1.5 mg/kg. Impact of MOK pharmacopuncture on body temperature and TRPV1 expression in hypothyroidism rats. To investigate the regulatory impact of body temperature in hypothyroidism, we measured the core body temperature, plus the expression in the thermoregulator, TRPV1 channel inside the DRG and brain tissues by western blot, respectively. In PTU-induced hypothyroidism rats, the physique temperature from two, three, and 4 weeks immediately after initial PTU remedy was significantly decrease than the normal group (P0.001) inside a time-dependent manner (Fig. 7A). MOK pharmacopuncture at 0.three and 1.five mg/kg resulted within a significantly (P0.01, respectively) greater physique temperature than that of the control group from 1 to two weeks just after initial treatment. Within the LT4-treated group, the body temperature was also drastically (P0.001) larger than these with the PTU handle group and typical rats. In LT-4-treated group, it was shown a considerable enhance of body temperature in hypothyroidism rats. The expression of TRPV1 was substantially decreased within the DRG (Fig. 7B) by MOK pharmacopuncture at 0.three (P0.01) and 1.five mg/kg (P0.05) and inside the brain at 0.four mg/kg (P0.01, Fig. 7C) of hypothyroidism rats compared with the typical group. The treatment of LT4 also significantly decreasedTRPV1 expression in each DRG (P0.01) and brain tissues (P0.01). Effects of MOK pharmacopunctureon the expression of IL4, IL10, Foxp3, and IFN within the spleen of hypothyroidism rats. To understand the action mechanism of MOK pharmacopuncture on Th1/Th2 immune response, we measured the serum levels of IFN-, Th1 cytokine, IL-4, and Th2 cytokine in hypothyroidism rats by ELISA plus the expression of IFN-, IL-4, IL-10, and Foxp3 mRNA within the spleen tissues by RT-PCR. Spleen weight was drastically (P0.01) decreased in hypothyroidism rats compared with that of the standard group, and this lower was substantially improved by MOK pharmacopuncture at 0.3 (P0.01) and 1.5 mg/kg (P0.01) or LT4 therapy (P0.05; Fig. 8A). Next, MOK pharmacopuncture considerably decreased at 0.three (P0.01) and 1.5 mg/kg (P0.01) inside the sera of hypothyroidism rats and significantly enhanced the IL-4 levels at 0.three (P0.01) and 1.five mg/kg (P0.05). MOK pharmacopuncture decreased the expression of IFN- mRNA, but enhanced the expression of IL-4 mRNA inside the spleen tissues of hypothyroidism rats (Fig. 8C). Further, MOK pharmacopuncture significantly elevated the expression of IL10 and Foxp3 mRNA within the spleen tissues of hypothyroidism rats. Discussion Pharmacopuncture is really a new type of acupuncture remedy in TKM; it’s also referred to as acupoint injection in TCM, andHWANG et al: EFFECTS OF MOK PHARMACOPUNCTURE ON HYPOTHYROIDISMFigure 7. Effect of MOK pharmacopuncture on the alterations in physique temperature and the expression of TRPV1 protein in PTU-induced hypothyroidism rats. MOK pharmacopuncture was subcutaneously administered as soon as daily for 2 weeks, as well as the physique temperature was measured by (A) rectal thermometer when a week. The production of TRPV1 protein was determined in (B) DRG and (C) brain tissues isolated from PTU-induced hypothyroidism rats using western blot. Data are presented as mean s.