Nt was shown to decrease the histopathological changes, including hyperplasia of follicular cells and associated hypertrophic modifications (Fig. 5A). Additionally, MOK pharmacopuncture at 0.three and 1.5 mg/kg drastically improved the follicular size (P0.001, respectively) compared with that on the control group (Fig. 5B).HWANG et al: EFFECTS OF MOK PHARMACOPUNCTURE ON HYPOTHYROIDISMFigure four. Effects of MOK pharmacopuncture 75330-75-5 custom synthesis around the modifications of serological parameters in PTU-induced hyperthyroidism rats. MOK pharmacopuncture was subcutaneously administered when each day for 2 weeks, as well as the levels of (A) glucose, (B) triglyceride, (C) total cholesterol, (D) LDL-cholesterol, (E) AST, and (F) ALT in the sera of rats have been PD1-PDL1-IN 1 Inhibitor measured by automatic blood biochemical analyzer. Data are presented as imply standard deviation (n=5 per every group). P0.05, P0.01, and P0.001 vs. normal; #P0.05, ##P0.01, and ###P0.001 vs. manage. Normal, normal group; PTU+Vehicle, handle group; PTU+Low MOK, MOK 0.three ml/kg-treated group in manage; PTU+High MOK, MOK 1.5 mg/kg-treated group in handle; and PTU+LT4, L-Thyroxine 0.five mg/kg-treated group as a reference drug.Figure 5. Effects of MOK pharmacopuncture on the histopathological alterations of thyroid tissues in PTU-induced hypothyroidism rats. MOK pharmacopuncture was subcutaneously administered once each day for 2 weeks, and thyroid glands were isolated in the rats. (A) Thyroid tissues have been stained with H E dye. Morphological changes were observed by a microscope at x200 in original magnification. Arrow: Follicle membrane, and f: Follicle. (B) The mean of relative follicular sizes to regular group have been measured in PTU-induced hypothyroidism rats. Data are presented as imply typical deviation (n=5 per every group). P0.001 vs. normal; ###P0.001 vs. handle. Typical, typical group; PTU+Vehicle, handle group; PTU+Low MOK, MOK 0.three ml/kg-treated group in handle; PTU+High MOK, MOK 1.five mg/kg-treated group in manage; and PTU+LT4, L-Thyroxine 0.five mg/kg-treated group as a reference drug.Effect of MOK pharmacopuncture on oxidation within the liver and brain of hypothroidism rats. To investigate the effect of MOK pharmacopuncture on oxidative harm in hypothyroidism, we measured the levels with the antioxidant substance GSH inside the liver tissues of hyperthyroidism rats along with the expression of the antioxidant enzymes SOD and CAT in both liver and brain tissues. As shown in Fig. 6A, the level ofGSH was significantly (P0.05) lowered within the liver tissues of PTUinduced hypothyroidism rats and substantially increased within the rats treated with MOK pharmacopuncture at 0.three (P0.01) and 1.five mg/kg (P0.05). Next, the expression of SOD protein was improved in hypothyroidism rats and drastically decreased in each liver (P0.05; Fig. 6B) and brain tissues (P0.01; Fig. 6C) compared with that from the handle group afterEXPERIMENTAL AND THERAPEUTIC MEDICINE 16: 310-320,Figure 6. Effect of MOK pharmacopuncture around the oxidation in liver and brain tissues of PTU-induced hypothyroidism rats. MOK pharmacopuncture was subcutaneously administered as soon as day-to-day for 2 weeks, as well as the levels of (A) GSH from the liver of rats by ELISA had been measured. The expression of CAT and SOD2 inside the (B) liver and (C) brain tissues employing western blot. Data are presented as imply regular deviation (n=5 per every single group). P0.05 vs. regular; # P0.05, ##P0.01, and ###P0.001 vs. control. Normal, regular group; PTU+Vehicle, manage group; PTU+Low MOK, MOK 0.three ml/kg-treated group in control; PTU+High MOK, MOK 1.5.