Biotinylated Human E-Selectin/CD62E Protein 5205

Additional Information:
express systemHEK293|product tagC-His-Avi|purity> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC|backgroundE-Selectin (Endothelial Leukocyte Adhesion Molecule-1, ELAM-1, CD62E), a member of the Selectin family, is a 107 115 kDa cell surface glycoprotein. It is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha.E-selectin mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with SELPLG/PSGL1. May have a role in capillary morphogenesis.|molecular weightThe protein has a predicted MW of 61.6 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Tris-Bis PAGE result.|available size100 g, 500 g|endotoxinLess than 1EU per g by the LAL method.|Biotinylated Human E-Selectin/CD62E Protein 5205proteinSize and concentration100, 500g and lyophilizedFormLyophilizedStorage InstructionsValid for 12 months from date of receipt when stored at -80C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.Storage bufferShipped at ambient temperature.Purity> 95% as determined by Tris-Bis PAGEtarget relevanceE-Selectin (Endothelial Leukocyte Adhesion Molecule-1, ELAM-1, CD62E), a member of the Selectin family, is a 107 – 115 kDa cell surface glycoprotein. It is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha.E-selectin mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with SELPLG/PSGL1. May have a role in capillary morphogenesis.Protein namesE-selectin (CD62 antigen-like family member E) (Endothelial leukocyte adhesion molecule 1) (ELAM-1) (Leukocyte-endothelial cell adhesion molecule 2) (LECAM2) (CD antigen CD62E)Gene namesSELE,SELE ELAM1Protein familySelectin/LECAM familyMass9606DaFunctionCell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with SELPLG/PSGL1. May have a role in capillary morphogenesis.Catalytic activityBINDING 101..109; /ligand=”a carbohydrate”; /ligand_id=”ChEBI:CHEBI:16646″; /evidence=”ECO:0000269|PubMed:26117840, ECO:0007744|PDB:4C16, ECO:0007744|PDB:4CSY”; BINDING 101; /ligand=”Ca(2+)”; /ligand_id=”ChEBI:CHEBI:29108″; /evidence=”ECO:0000269|PubMed:26117840, ECO:0007744|PDB:1ESL, ECO:0007744|PDB:4C16, ECO:0007744|PDB:4CSY”; BINDING 103; /ligand=”Ca(2+)”; /ligand_id=”ChEBI:CHEBI:29108″; /evidence=”ECO:0000269|PubMed:11081633, ECO:0000269|PubMed:26117840, ECO:0007744|PDB:1ESL, ECO:0007744|PDB:1G1T, ECO:0007744|PDB:4C16, ECO:0007744|PDB:4CSY”; BINDING 109; /ligand=”Ca(2+)”; /ligand_id=”ChEBI:CHEBI:29108″; /evidence=”ECO:0000269|PubMed:11081633, ECO:0000269|PubMed:26117840, ECO:0007744|PDB:4C16, ECO:0007744|PDB:4CSY”; BINDING 113..118; /ligand=”a carbohydrate”; /ligand_id=”ChEBI:CHEBI:16646″; /evidence=”ECO:0000269|PubMed:26117840, ECO:0007744|PDB:4C16, ECO:0007744|PDB:4CSY”; BINDING 126..128; /ligand=”a carbohydrate”; /ligand_id=”ChEBI:CHEBI:16646″; /evidence=”ECO:0000269|PubMed:26117840, ECO:0007744|PDB:4C16, ECO:0007744|PDB:4CSY”; BINDING 126; /ligand=”Ca(2+)”; /ligand_id=”ChEBI:CHEBI:29108″; /evidence=”ECO:0000269|PubMed:11081633, ECO:0000269|PubMed:26117840, ECO:0007744|PDB:1ESL, ECO:0007744|PDB:1G1T, ECO:0007744|PDB:4C16, ECO:0007744|PDB:4CSY”; BINDING 127; /ligand=”Ca(2+)”; /ligand_id=”ChEBI:CHEBI:29108″; /evidence=”ECO:0000269|PubMed:11081633, ECO:0000269|PubMed:26117840, ECO:0007744|PDB:1ESL, ECO:0007744|PDB:1G1T, ECO:0007744|PDB:4C16, ECO:0007744|PDB:4CSY”Subellular locationCell membrane ; Single-pass type I membrane protein.StructureInteracts with SELPLG/PSGL1 and PODXL2 through the sialyl Lewis X epitope. SELPLG sulfation appears not to be required for this interaction.Target Relevance information above includes information from UniProt accession: P16581The UniProt Consortium|