Mouse MEPE Protein 3987

Additional Information:
inaccessionQ8K4L6|express systemHEK293|product tagC-His|purity> 95% as determined by Tris-Bis PAGE|backgroundMatrix extracellular phosphoglycoprotein (MEPE) is expressed in bone and teeth where it has multiple functions. The C-terminus of MEPE contains a mineral-binding, acidic serine- and aspartate-rich motif (ASARM) that is also present in other noncollagenous proteins of mineralized tissues.MEPE-derived ASARM peptides function in phosphate homeostasis and direct inhibition of bone mineralization in a phosphorylation-dependent manner.|molecular weightThe protein has a predicted MW of 45.3 kDa. The protein is cleaved into 2 chains and due to glycosylation, it migrates to 45-60 kDa based on Tris-Bis PAGE result.|available size100 g, 500 g|endotoxinLess than 1EU per g by the LAL method.|Mouse MEPE Protein 3987proteinSize and concentration100, 500g and lyophilizedFormLyophilizedStorage InstructionsValid for 12 months from date of receipt when stored at -80C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.Storage bufferShipped at ambient temperature.Purity> 95% as determined by Tris-Bis PAGEtarget relevanceMatrix extracellular phosphoglycoprotein (MEPE) is expressed in bone and teeth where it has multiple functions. The C-terminus of MEPE contains a mineral-binding, acidic serine- and aspartate-rich motif (ASARM) that is also present in other noncollagenous proteins of mineralized tissues.MEPE-derived ASARM peptides function in phosphate homeostasis and direct inhibition of bone mineralization in a phosphorylation-dependent manner.Protein namesMatrix extracellular phosphoglycoprotein (Osteoblast/osteocyte factor 45) (OF45) (Osteoregulin)Gene namesMepe,MepeProtein familyPF07175/osteoregulin familyMass10090DaFunctionRegulates renal phosphate and uric acid excretion (PubMed:26051469). Regulates bone mineralization by osteoblasts and cartilage mineralization by chondrocytes (PubMed:11414762, PubMed:12421822, PubMed:15843468, PubMed:22766095). Regulates the mineralization of the extracellular matrix of the craniofacial complex, such as teeth, bone and cartilage (PubMed:26927967). Increases dental pulp stem cell proliferation (By similarity).Subellular locationSecreted, extracellular space, extracellular matrix. Secreted .TissuesExpressed in osteocytes (at protein level) (PubMed:12421822, PubMed:15221418). Expressed by chondrocytes, specifically in the hypertrophic zone of the bone growth plate (at protein level) (PubMed:22766095). Expressed in osteoblasts in bone (at protein level) (PubMed:11414762, PubMed:15221418, PubMed:18597632). Expressed by osteoblasts within the metaphysis (at protein level) (PubMed:15221418, PubMed:22766095). Expressed at low levels in white fat, brown fat, testes, brain and aorta (PubMed:12421822). Expressed in the craniofacial complex (at protein level) (PubMed:26927967). Expressed in odontoblasts, ameloblasts and in predentin during tooth development (at protein level) (PubMed:22042093). Expressed in the kidney (at protein level) (PubMed:26051469). Expressed in osteocytes in mandibular condylar cartilage and tibial cartilage (at protein level) (PubMed:26428891). Expressed in salivary glands (PubMed:15329369).StructureInteracts (via ASARM motif) with PHEX; the interaction is zinc-dependent.Post-translational modificationPhosphorylated on serine residues in the ASARM motif; the phosphorylation is important for the inhibition of bone mineralization.; Cleaved by CTSB/cathepsin B; the cleavage is blocked by metalloprotease PHEX.DomainThTarget Relevance information above includes information from UniProt accession: Q8K4L6The UniProt Consortium|