Name :
DDR1 (Human) Recombinant Protein
Biological Activity :
Human DDR1(Q08345-1, Asp21-Ala417) partial recombinant protein with His tag at C-terminus expressed in HEK293 cells.Bioactive Protein,Bioactive Proteins,Bioactive,Active,Functional Protein,Functional Proteins,Recombinant Human Protein,Recombinant Human Proteins,Human Recombinant Protein,Human Recombinant Proteins,HuPro
Tag :
Result of bioactivity analysis
Protein Accession No. :
Q08345-1
Protein Accession No.URL :
https://www.ncbi.nlm.nih.gov/gene?cmd=Retrieve&dopt=Graphics&list_uids=780
Amino Acid Sequence :
Asp21-Ala417
Molecular Weight :
45.1
Storage and Stability :
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.Aliquot to avoid repeated freezing and thawing.
Host :
Human
Interspecies Antigen Sequence :
Preparation Method :
Mammalian cell (HEK293) expression system
Purification :
Quality Control Testing :
SEC-HPLC and Tris-Bis PAGE SEC-HPLC The purity of Human DDR1 is greater than 95% as determined by SEC-HPLC. Tris-Bis PAGE Human DDR1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Storage Buffer :
Lyophilized from sterile distilled Water is > 100 ug/mL
Applications :
Enzyme-linked Immunoabsorbent Assay， Immobilized Human DDR1, His Tag at 0.5 ug/mL (100 uL/well) on the plate. Dose response curve for Anti-DDR1 Antibody, hFc Tag with the EC50 of 5.9 ng/mL determined by ELISA (QC Test). Functional Study， SDS-PAGE， Surface Plasmon Resonance， Human DDR1, His Tag captured on CM5 Chip via Anti-His Antibody can bind Native Human Collagen I protein with an affinity constant of 0.806 nM as determined in SPR assay (Biacore T200).
Gene Name :
DDR1
Gene Alias :
CAK, CD167, DDR, EDDR1, MCK10, NEP, NTRK4, PTK3, PTK3A, RTK6, TRKE
Gene Description :
discoidin domain receptor tyrosine kinase 1
Gene Summary :
Receptor tyrosine kinases (RTKs) play a key role in the communication of cells with their microenvironment. These molecules are involved in the regulation of cell growth, differentiation and metabolism. The protein encoded by this gene is a RTK that is widely expressed in normal and transformed epithelial cells and is activated by various types of collagen. This protein belongs to a subfamily of tyrosine kinase receptors with a homology region to the Dictyostelium discoideum protein discoidin I in their extracellular domain. Its autophosphorylation is achieved by all collagens so far tested (type I to type VI). In situ studies and Northern-blot analysis showed that expression of this encoded protein is restricted to epithelial cells, particularly in the kidney, lung, gastrointestinal tract, and brain. In addition, this protein is significantly over-expressed in several human tumors from breast, ovarian, esophageal, and pediatric brain. This gene is located on chromosome 6p21.3 in proximity to several HLA class I genes. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq
Other Designations :
OTTHUMP00000029343|OTTHUMP00000029344|OTTHUMP00000029345|OTTHUMP00000029346|OTTHUMP00000029347|PTK3A protein tyrosine kinase 3A|cell adhesion kinase|discoidin domain receptor DDR1d|discoidin domain receptor family, member 1|discoidin receptor tyrosine kin
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