As proven in Fig. seven, antibody subtype differentiation was achievable the two with the polyclonal rabbit sera (Fig. 7A-C) and with serum derived from a chicken that had been vaccinated from H5N9 (Fig 7D) in the indirect ELISA structure, while in the blocking ELISA format no differentiation was attainable (data not shown)
In Western blot analyses, all 3 sera recognized all three antigens. In a next...