Egree worth of 89 and aInt. J. Mol. Sci. 2018, 19,six ofThe network reports a scalefree topology, indicating that extremely connected Ramoplanin Data Sheet proteins are positioned Def Inhibitors products within the central region, as indicated in Figure 3B. After analyzing the degree of connectivity by variety of Figure 3. (A) Interaction network of T. cruzi strain CL Breiner. The nodes (proteins) are presented in nodes, we found that this distribution had a linear tendency behavior using a negative slope, with a R2 black plus the score linked with all the interaction is presented in colors, blue getting much more trustworthy of 0.735 (unpublished figure). Despite the fact that this is not a higher value in comparison with other networks (probably than orange. (B) Interaction network of T. cruzi strain CL Breiner. The axes (interactions) are because of lack of coverage), and onsufficient condition toconnected)with posterior topological calculations. it is actually a a scale of orange (significantly less proceed to blue (much more connected) the nodes represented in black We also observedthelarge numbersizenodescircle indicates its degree connectivity (Figure 3B). (proteins) of a network. The of of the having a high degree of of connectivity (degree). 2.five. Identification of Proteins Potentially Involved within the AKTLike Pathway in the Parasite 2.5. Identification of Proteins Potentially Involved within the AKTLike Pathway in the Parasite Immediately after obtaining a listlist human proteins linked towards the PI3KAKTmTOR pathway, we mapped Soon after obtaining a of of human proteins related for the PI3KAKTmTOR pathway, we them with them with proteinsthe protein rotein protein rotein interaction network of TcAKTlike mapped proteins incorporated in incorporated in the interaction network of T. cruzi, including T. cruzi, (Figure 4A).TcAKTlike (Figure 4A). The complete results are reported inTopological properties were including The comprehensive results are reported in Supplementary Table S3. Supplementary Table S3. calculated, exactly where the TcAKTlike yielded where the TcAKTlike yielded a degree worth of 89 and a Topological properties were calculated, a degree value of 89 and a betweenness centrality of 0.002, which might be associated of a pleiotropic nature.linked proteins had been also analyzed othervisualized betweenness centrality to 0.002, which could be The other to a pleiotropic nature. The and proteins as bluealso analyzed and visualized as blue nodes respective4B. The proteins with their respective were nodes in Figure 4B. The proteins with their in Figure calculations of degrees of connectivity (degree) and betweenness connectivity (degree) and Table S4. calculations of degrees of centrality are reported in betweenness centrality are reported in Table S4.Figure (A) Protein rotein interaction network with all the AKTlike protein signaled in yellow, where Figure 4.4. (A) Protein rotein interaction network with all the AKTlike protein signaled in yellow, where the axes that connect using the closest neighboring nodes (located inside the center of network) are the axes that connect with all the closest neighboring nodes (positioned in the center of your the network) are highlighted in red. (B) Protein rotein interaction network with proteins represented as nodes, highlighted in red. (B) Protein rotein interaction network with proteins represented as white white nodes, and in blue are were that have been mapped with the PI3KAKTmTOR human of human and in blue are these that those mapped using the PI3KAKTmTOR pathway ofpathwayproteins. proteins.Most of the proteins reported connectivity information within the imply and are also rela.