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Left to PDMS layer and bond the PDMS film to to uncured PDMS layer.to crosslink the uncured rest in the ambient temperature for 48 h the array on the uncured PDMS layer and bond the PDMS film to the array of strained holes. crosslinkstrained holes.Micromachines 2021, 12, x FOR PEER REVIEW4 ofFigure 1. The tool made use of for stretching the sample. Figure 1. The tool employed for stretching the sample.The assembly was sprayed with SU-8 developer (Microchem Newton, MA, USA), which resulted in dissolution from the unexposed photoresist film that lay between the Polmacoxib Autophagy crossClamping Stretching Gluing Releasing linked PDMS film and also the BOPET film, and therefore removal of the BOPET film. The surface of the PDMS film was rinsed successively with fresh developer and deionized water ahead of drying. Ultimately, the displacements applied towards the PDMS sheet had been released in each planar directions simultaneously, which produced an array of curved film microstructures. The forming approach of film microstructures corresponding towards the fabrication methods is shown in Figure 2b. The MRTX-1719 Epigenetic Reader Domain two-dimensional (2D) morphology of the curved film microstructures was assessed by using an optical microscope (Olympus STM6-F10-3, Olympus Co., Tokyo, Ja(b) pan), while the 3D morphology of the curved film microstructures was assessed by utilizing a laser scanning confocal microscope (Nikon A1, gold-coated, Nikon, Tokyo, Japan). The 2D cross-sectional view in the curved film microstructures was examined by optical microscope (Nikon SMZ1270, colored film microstructures, Nikon, Tokyo, Japan). The 2D Figure 2. (a) Fabrication process of curved film microstructure array; (b) Forming method of film microstructures surface profile of a common curved microstructure was characterized by profiler corresponding to fabrication measures. (VeecoDektak 150, Veeco, Plainview, NY, USA). Figure two. (a) Fabrication process of curved filmwas sprayed with SU-8 Forming procedure of film microstructures cor-USA), The assembly microstructure array; (b) developer (Microchem, Newton, MA, responding to fabrication steps. which resulted in dissolution on the unexposed photoresist film that lay amongst the crosslinked PDMS film plus the BOPET film, and therefore removal of your BOPET film. The sur3. Final results and Discussion rinsed successively with fresh developer and deionized water face with the PDMS film was Figure 3a,b display the 2D morphology on the the PDMS sheet have been released array. before drying. Ultimately, the displacements applied to fabricated film microstructure in both The 2D profiles appear really uniform, showing a circular shape with afilm microstructures. planar directions simultaneously, which developed an array of curved diameter of around 250 m, which is nearly equal for the diameter from the holes of the PDMS sheet. is shown The forming procedure of film microstructures corresponding towards the fabrication actions The 3D in Figure 2b. surface topography from the film microstructures is presented in Figure 3c, along with the 2D crossThe two-dimensional (2D) morphology from the curved film 3d. Apart from very good unisectional view on the film microstructures is presented in Figuremicrostructures was assessed by using an optical microscopethe flat film in the bottom on the microstructures is formity, the smooth connection with (Olympus STM6-F10-3, Olympus Co., Tokyo, Japan), whilst the 3D morphology The 2D surface profile of a common curved film by utilizing a laser observed from the figures. in the curved film microstructures was assessed microstructure scanning c.

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