L Appl Pharmacol. Author manuscript; NK1 Agonist drug available in PMC 2015 September 15.Gilbert et al.PageNIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author ManuscriptFigure 2. TCE inhibits macrophage Il6 expression in dose-dependent mannerCytokine gene expression was examined in peritoneal macrophages incubated with (open bars) or with no LPS (shaded bars) right after isolation from untreated handle mice or from mice exposed to TCE for 12 weeks. The data represents the imply SD. Significantly distinct (0.05) in comparison to control values.Toxicol Appl Pharmacol. Author manuscript; readily available in PMC 2015 September 15.Gilbert et al.PageNIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author ManuscriptToxicol Appl Pharmacol. Author manuscript; out there in PMC 2015 September 15.Figure three. TCE inhibition IL-6 production is maintained more than timePeritoneal macrophages have been incubated with LPS following isolation from untreated manage mice or from mice exposed to TCE (0.five mg/ml) for as much as 40 weeks. Culture supernatants were examined for cytokines (mean SD). Considerably unique (0.05) when compared with handle values.Gilbert et al.PageNIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author ManuscriptFigure four. TCE inhibition of Il6 expression is maintained over timeCytokine gene expression was examined in peritoneal macrophages incubated with or with no LPS right after isolation from untreated control mice or from mice exposed to TCE (0.five mg/ml) for as much as 40 weeks. The data represents the imply SD. Drastically MMP-7 Inhibitor Biological Activity different (0.05) in comparison to handle values.Toxicol Appl Pharmacol. Author manuscript; out there in PMC 2015 September 15.Gilbert et al.PageNIH-PA Author ManuscriptFigure five. TCE alters expression of hepatic genes over timeA. Gene expression in person liver tissue isolated from untreated manage mice or from mice exposed to TCE (0.5 mg/ml) for as much as 40 weeks. The information represents the imply SD from six person mice/treatment/time point. Significantly unique (0.05) when compared with handle values. B. Relative protein levels (percentage reference protein GAPDH) of IL-6R in individual livers from untreated control mice or mice exposed to TCE (0.five mg/ml) for 16 weeks (imply SD).NIH-PA Author Manuscript NIH-PA Author ManuscriptToxicol Appl Pharmacol. Author manuscript; obtainable in PMC 2015 September 15.Gilbert et al.PageNIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author ManuscriptFigure 6. TCE liver pathology correlates with loss of hepatic Il-6r expressionA. Liver pathology based on immune cell infiltration and inflammation was assessed in mice exposed to TCE (0.5mg/ml) for 28, 34 or40 weeks. B. Equal amounts of liver protein from an untreated mouse had been separated in four lanes of SDS-PAGE, each of which had been immunoblotted with pooled sera obtained from control MRL+/+ mice or mice treated with 0.5 mg/ml TCE for four or 40 weeks. C. Hepatic gene expression in from mice exposed to TCE (0.five mg/ml) for 40 weeks was plotted against liver histopathology in the exact same mice. Gene expression values are shown in log scale as a result of proper skewness. Regression p-values had been computed employing an F test in the null hypothesis of horizontal slope.Toxicol Appl Pharmacol. Author manuscript; available in PMC 2015 September 15.Gilbert et al.PageNIH-PA Author Manuscript NIH-PA Author ManuscriptFigure 7. Submodel for parameter estimationA. An IL-6 submodel was developed for estimating dose-dependent reduction in the fraction of IL-6 expressed by the macrophage. Point.
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